Journal: Pharmaceuticals

Article Title: The Pharmacological TAILS of Matrix Metalloproteinases and Their Inhibitors

doi: 10.3390/ph14010031

Figure Lengend Snippet: MMPs inhibitors in clinical trials.

Article Snippet: Selective MT1-MMP inhibitors alone or in combination with Tamiflu ® also demonstrated efficacy in influenza infection models and could be further investigated for other viral infections.

Techniques:

Journal: Pharmaceuticals

Article Title: The Pharmacological TAILS of Matrix Metalloproteinases and Their Inhibitors

doi: 10.3390/ph14010031

Figure Lengend Snippet: Small molecule exosite MMP inhibitors.

Article Snippet: Selective MT1-MMP inhibitors alone or in combination with Tamiflu ® also demonstrated efficacy in influenza infection models and could be further investigated for other viral infections.

Techniques: Binding Assay, Inhibition, Migration, Activation Assay, In Vitro, Synthesized, Activity Assay

Journal: Pharmaceuticals

Article Title: The Pharmacological TAILS of Matrix Metalloproteinases and Their Inhibitors

doi: 10.3390/ph14010031

Figure Lengend Snippet: Regulation of MMP inhibition by antibodies.

Article Snippet: Selective MT1-MMP inhibitors alone or in combination with Tamiflu ® also demonstrated efficacy in influenza infection models and could be further investigated for other viral infections.

Techniques: Inhibition, Binding Assay, Activity Assay

Journal: Science Advances

Article Title: An injury-responsive mmp14b enhancer is required for heart regeneration

doi: 10.1126/sciadv.adh5313

Figure Lengend Snippet: ( A ) Schematic of the experimental design for neonatal mouse heart injury and MMP-14 inhibitor treatment. Myocardial injury by coronary artery ligation was conducted on P1, mice were simultaneously treated with a single intraperitoneal injection of 20 μM NSC405020 or DMSO (vehicle control), and hearts were collected on P4 for analysis by Western blot (WB). ( B ) Western blots for Agrin and β-actin from total heart tissue 3 dpi. ( C ) Quantification of the 260-kD isoform of Agrin protein normalized to β-actin and expressed in AU in neonatal mouse hearts following myocardial injury (3 dpi) and treatment with either DMSO or NSC405020. Statistical significance was determined using Student’s t test. * P < 0.05.

Article Snippet: For inhibitor studies, following amputation, zebrafish were divided into 500-ml tanks, the MT1-MMP inhibitor NSC405020 (Sigma-Aldrich) was added in DMSO at a final inhibitor concentration of 20 μM, and caudal fin regeneration in the presence of inhibitor, compared to the addition of 0.1% DMSO alone, was assessed at the indicated time points.

Techniques: Ligation, Injection, Western Blot

Journal: Molecular Cancer Research

Article Title: Syntaxin4-Munc18c Interaction Promotes Breast Tumor Invasion and Metastasis by Regulating MT1-MMP Trafficking

doi: 10.1158/1541-7786.MCR-20-0527

Figure Lengend Snippet: Invadopodium formation and gelatin degradation in MDA-MB-231 cells is MT1-MMP–dependent. A, Parental MDA-MB-231 cells and stable cells expressing Stx4-FL, Stx4-N-term, Stx4-1–15, and Stx4-15–29 were treated with either DMSO (control) or MAB3329 and NSC405020, plated onto gelatin for 4 hours, then fixed, permeabilized, stained for F-actin, and analyzed by confocal microscopy. B, Cells, as in A, were plated onto gelatin for 24 hours, then fixed, permeabilized, stained for F-actin, and analyzed by confocal microscopy. Invadopodia ( A ) and gelatin degradation ( B ) were quantified as described in “Materials and Methods”. Means ± SD are presented from three or more biological replicates, with at least three technical replicates; asterisks denoting values significantly different from controls (*, P < 0.05).

Article Snippet: MT1-MMP inhibitors MAB3329 and NSC 405020 were purchased from Millipore Sigma and Tocris Biotechne, respectively.

Techniques: Expressing, Control, Staining, Confocal Microscopy